Table 1.
Characteristics of designed sgRNAs and their activity.
| Name of sgRNA | 20-nt Base-Pairing Region | Tm, °C | GC, % | Average Repression Range, Fold ± SE |
|---|---|---|---|---|
| fur-sg1 | ACCTTCTAGGTTTGGTGATT | 58.5 | 40 | 23 ± 0.6 |
| fur-sg2 | ACGAATCAACAAGATTGAGT | 57.4 | 35 | 43 ± 8.8 |
| whiA-sg1 | CATTTTGCACCAACAAGTCG | 60.9 | 45 | 3 ± 0.2 |
| whiA-sg2 | AGATGATCGTTTTTGGGTGT | 59.9 | 40 | 33 ± 8.3 |
| spxA-sg1 | ATAATACTATATTGTTCGTC | 49.4 | 25 | 9 ± 0.2 |
| spxA-sg2 | GTTATTTTGATTATCTGTCA | 50.7 | 25 | 0.7 ± 0.02 |
| hup1-sg1 | AAACAAGCCTTAACAAGTTT | 56.2 | 30 | 3 ± 0.1 |
| hup1-sg2 | ACTCTAAAAGTACCAAGTTC | 54.5 | 35 | 22 ± 2.8 |
| deoA-sg1 | AAAGCGGCAGCTTGGTAGTC | 65.6 | 55 | 11 ± 1.2 |
| deoA-sg2 | GCCTGAATGCATCATAGCGT | 63.3 | 50 | 42 ± 8.5 |