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. 2022 Jun 6;15(6):721. doi: 10.3390/ph15060721

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vitro radioprotective effects evaluation of CY-TMP conjugates. (a) Cytotoxicity evaluation of CY-TMP1, CY-TMP2, CY-TMP3 and Amifostine on L-02 cells. “***” represents significant difference from 0 μM group, “ns” represents no significance. *** p < 0.001, Data are expressed as Mean ± SD (n = 3). (b) Radioprotective effect of CY-TMP1 on normal cells (L-02) and cancer cells (B16-F10). CY-TMP1 selectively protects normal cells rather than cancer cells from ionizing radiation injury. Comparison of cell viability after treatment with CY-TMP1 (25 μM) between radiation and non-radiation groups, n = 3, ** p < 0.01, “ns” represents no significance. (c) Effect of CY-TMP1 (25 μM) on the colony formation ability of L-02 cells in the absence or presence of X-ray irradiation (6 Gy).