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. 2022 Jun 6;15(6):721. doi: 10.3390/ph15060721

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mechanism study of CY-TMP1 with the radioprotection effect. (a) Subcellular localization of CY-TMP1 was detected with a mitochondria-specific green probe (Mito-Tracker); (b) Alterations in mitochondrial membrane potential (MMP) and mitochondrial ROS (Mito-ROS) were detected by a fluorescence microscope before and after 6 Gy X-ray radiation; (c) Quantitative analysis of mito-ROS based on the mean fluorescent intensity of per filed with Image J software (n = 3, mean ± SD, ** p < 0.01, versus CY-TMP1+6 Gy group); (d) ATP was determined and compared with or without pretreatment of CY-TMP1, (n = 3, mean ± SD, * p < 0.05, ** p < 0.01).