Figure 1.

Mortality rates with 95% confidence intervals of vaccinated broiler chickens following necrotic enteritis challenges. Birds were immunized with recombinant proteins on Day 4 and Day 15: EMCP = positive challenge control; Adj = adjuvant + EMCP challenge; NA= chimeric NetB and Plc; FZ = chimeric FBA and Zm; Cna = collagen-binding protein; Pooled = pooled NA, FZ, and Cna antigens. The birds with 13 birds per group were then challenged with Eimeria maxima (EM, 5 × 10³ oocysts/bird, oral gavage) on Day 22, followed by CP netB⁺tpel⁺ LLY_Tpel 17 (1 × 10⁹ cfu /bird, oral gavage) on Day 26. Mortalities were recorded after C. perfringens challenge.