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. 2022 Jun 20;10(6):979. doi: 10.3390/vaccines10060979

Figure 2.

Figure 2

Lesion scores in vaccinated broiler chickens following necrotic enteritis challenge. The statistical differences were analyzed by the Kruskal–Wallis test and the Mann–Whitney U test. The chicks with 13 birds per group were immunized with recombinant proteins on Day 4 and Day 15: EMCP = positive challenge control; Adj = adjuvant + EMCP challenge; NA = chimeric NetB and Plc; FZ= chimeric FBA and Zm; Cna= collagen-binding protein; Pooled = pooled NA, FZ, and Cna antigens. The vaccinated birds were then administrated with Eimeria maxima (EM, 5 × 103 oocysts/bird, oral gavage) on Day 22, followed by C. perfringens (CP) netB+tpel+LLY_Tpel 17 strain (1 × 109 cfu /bird, oral gavage) on Day 26. Birds were sacrificed on Day 2 post-CP infection, and jejunum lesion scores were determined. The * or # marks show statistically significant difference (* p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001) when compared with the value in Adj group, while # p ≤ 0.05; ## p ≤ 0.01; #### p ≤ 0.0001 when compared with the value in EMCP group, respectively).