Table 1.
Experimental design of vaccination studies *.
| Group No. | Group Name | Bird Number |
Vaccination (D4, D15) |
Challenge | Sacrifice (D28) |
|
|---|---|---|---|---|---|---|
| EM(D22) | CP (D26) | |||||
| 1 | Naive | 13 | PBS | PBS | BYC | S |
| 2 | Challenge Control (EMCP) | 13 | PBS | EM | CP | S |
| 3 | Adjuvant control (Adj) |
13 | Adj + PBS | EM | CP | S |
| 4 | Cna | 13 | Adj + Cna | EM | CP | S |
| 5 | FZ | 13 | Adj + FZ | EM | CP | S |
| 6 | NA | 13 | Adj + NA | EM | CP | S |
| 7 | Pooled (NA + FZ + Cna) |
13 | Adj + NA + FZ + Cna | EM | CP | S |
* Vaccinated chickens were challenged with E. maxima (EM, 5 × 103 oocysts/bird orally on Day 22), followed by oral gavage of C. perfringens (CP, 1 × 109 CFU at D26). BYC = BYC medium; PBS = phosphate-buffered saline; S = sacrificed; EMCP = positive challenge control; Adj = adjuvant + EMCP challenge; NA= chimeric NetB and Plc; FZ = chimeric FBA and Zm; Cna = collagen-binding protein; Pooled = pooled NA, FZ, and Cna antigens.