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The design of the HA stem strategy and key candidates. (A) Schematic of full length, B60-Stem and B60-Stem-CR8071 HA (B/Brisbane/60/2008(Victoria lineage); PDB:4FQM). (B) The prediction model of each design phase by AlphaFold2. GSA linker was colored in red, CR8033 epitope (blue), 12G6 epitope (green), SD84 epitope (purple), 5A7 epitope (orange) and CR8071 epitope (yellow) (drawn by PyMOL modeling software). (C) Ribbon HA stem model with color-coded modifications.
