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. 2022 Jun 14;14(6):1305. doi: 10.3390/v14061305

Figure 3.

Figure 3

Characterization and conformational determination of B60-Stem-8071 HA. (A) The image of SDS-PAGE and the Western blot of purified B60-Stem-8071. (B) The HPLC analysis of B60-Stem-8071. B60-Stem-8071 under reducing condition (black) and non-reducing condition (red). (C) Binding curves of broadly neutralizing antibodies CR8071 (in red), CR9114 (in blue) and negative (NC) group (non-influenza antibody in grey). ELISA reactivity to purified B60-Stem-8071 (D) using a competition ELISA assay with two antisera (B60-stem-8071 and NC). CR8071 was used as a competitor, and NC group mice vaccinated with non-influenza protein. The data represented the average of four independent experiments and error bars indicated the standard errors of the means (SEM), with asterisks representing significant differences (*** p < 0.001).