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. 2022 Jun 14;14(6):1305. doi: 10.3390/v14061305

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Reactivity of sera from mice vaccinated with B60-stem 8071 against divergent IBV in vitro. (A) Broad binding activity of sera from mice vaccinated with B60-stem-8071 against representative inactivated viruses from three influenza B lineages and an IAV pdmH1N1 strain by ELISA. (B) Neutralization activity of sera from mice vaccinated with B60-stem-8071 or adjuvant (NC group) to IBV by MN assay. The neutralization strength is color coded from green (weak) to red (strong). The value <10: no reactivity (filled with green); 10–40: moderate reactivity (filled with yellow); >40: high reactivity (filled with red). Asterisks represented significant differences Significance (* p < 0.05). (C)The ADCC effect of sera from mice vaccinated with B60-stem-8071 or NC group.