Table 1.
Effect of 2,4-D alone or in combination with BA or Kin on callus induction, fresh, and dry weight of callus from internodes after 6 weeks of culture.
| PGRs (μM) | Response % | FW (mg) | DW (mg) |
|---|---|---|---|
| 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| 1.0 µM 2,4-D | 71.23 ± 0.74 f | 92.11 ± 1.66 i | 9.66 ± 0.21 h |
| 2.5 µM 2,4-D | 79.61 ± 0.75 e | 192.15 ± 0.99 g | 18.82 ± 0.44 g |
| 5.0 µM 2,4-D | 91.23 ± 0.82 c | 285.35 ± 1.52 f | 26.12 ± 0.62 f |
| 10 µM 2,4-D | 94.46 ± 0.83 b | 391.15 ± 2.24 e | 37.45 ± 0.69 e |
| 20 µM 2,4-D | 79.20 ± 1.69 e | 178.12 ± 4.4 h | 16.43 ± 0.47 h |
| 10 µM 2,4-D + 1.0 µM BA | 97.82 ± 0.86 a | 672.52 ± 0.23 a | 68.61 ± 0.51 a |
| 10 µM 2,4-D + 2.5 µM BA | 95.66 ± 1.36 b | 484.03 ± 1.11 b | 49.24 ± 0.59 b |
| 10 µM 2,4-D + 1.0 µM Kin | 81.03 ± 0.89 e | 372.12 ± 1.94 d | 39.02 ± 0.99 d |
| 10 µM 2,4-D + 2.5 µM Kin | 84.65 ± 1.44 d | 429.76 ± 2.93 c | 45.14 ± 1.36 c |
Values are means ± SEM, n = 20 per treatment group. Means in a column without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the Duncan test.