Table 3.
Effect of auxins (IAA or IBA) with optimal concentrations of BA (5.0 μM) and Kin (5.0 μM) on shoot multiplication in callus mass obtained from internodal explants after 8 weeks of culture.
| BA | Kin | NAA | IAA | IBA | Response % | Mean Number of Shoots | Mean Shoot Length (cm) |
|---|---|---|---|---|---|---|---|
| 5.0 | - | 0.0 | - | - | 85.54 ± 0.58 d | 30.42 ± 0.51 jkl | 3.90 ± 0.11 bf |
| 5.0 | - | 0.5 | - | - | 90.22 ± 0.67 c | 41.23 ± 0.86 bc | 3.74 ± 0.11 defg |
| 5.0 | - | 1.0 | - | - | 98.44 ± 0.51 a | 54.63 ± 1.54 a | 4.54 ± 0.05 a |
| 5.0 | - | 1.5 | - | - | 92.48 ± 0.69 b | 42.84 ± 1.16 b | 4.26 ± 0.08 ab |
| 5.0 | - | 2.0 | - | - | 79.23 ± 0.62 ef | 28.47 ± 0.92 km | 3.58 ± 0.15 efh |
| 5.0 | - | - | 0.5 | - | 79.64 ± 0.51 ef | 33.42 ± 1.03 fi | 3.24 ± 0.18 hijk |
| 5.0 | - | - | 1.0 | - | 91.73 ± 0.71 bc | 43.67 ± 1.21 b | 4.32 ± 0.10 ab |
| 5.0 | - | - | 1.5 | - | 85.64 ± 0.62 d | 37.23 ± 1.24 de | 3.94 ± 0.27 be |
| 5.0 | - | - | 2.0 | - | 79.54 ± 0.69 ef | 27.81 ± 0.97 lm | 3.36 ± 0.18 ghij |
| 5.0 | - | - | - | 0.5 | 76.41 ± 0.89 gh | 30.25 ± 0.73 jkl | 3.04 ± 0.22 jl |
| 5.0 | - | - | - | 1.0 | 84.83 ± 0.66 d | 39.46 ± 0.51 cd | 4.04 ± 0.05 bd |
| 5.0 | - | - | - | 1.5 | 77.85 ± 0.86 fg | 31.81 ± 1.24 ghij | 2.96 ± 0.05 jl |
| 5.0 | - | - | - | 2.0 | 69.87 ± 0.86 ij | 26.81 ± 1.53 m | 2.78 ± 0.11 l |
| - | 5.0 | 0.5 | - | - | 79.67 ± 0.92 ef | 33.86 ± 0.86 fh | 3.57 ± 0.070 fi |
| - | 5.0 | 1.0 | - | - | 90.95 ± 0.71 c | 41.82 ± 1.28 bc | 4.37 ± 0.083 ab |
| - | 5.0 | 1.5 | - | - | 81.21 ± 0.86 e | 34.80 ± 1.16 ef | 3.94 ± 0.068 be |
| - | 5.0 | 2.0 | - | - | 75.57 ± 0.56 h | 30.83 ± 0.66 ikl | 3.48 ± 0.13 fi |
| - | 5.0 | - | 0.5 | - | 75.46 ± 0.47 h | 30.07 ± 0.83 jkl | 3.08 ± 0.12 il |
| - | 5.0 | - | 1.0 | - | 84.62 ± 0.68 d | 35.71 ± 0.70 ef | 4.18 ± 0.10 abc |
| - | 5.0 | - | 1.5 | - | 80.12 ± 0.63 e | 31.23 ± 0.58 hik | 3.84 ± 0.22 cdef |
| - | 5.0 | - | 2.0 | - | 70.76 ± 0.58 i | 28.68 ± 0.74 km | 3.12 ± 0.12 il |
| - | 5.0 | - | - | 0.5 | 70.36 ± 0.54 ij | 29.48 ± 0.67 jkm | 2.98 ± 0.14 jl |
| - | 5.0 | - | - | 1.0 | 81.29 ± 0.67 e | 34.61 ± 0.51 efg | 3.94 ± 0.12 be |
| - | 5.0 | - | - | 1.5 | 71.84 ± 1.04 i | 31.23 ± 0.66 hik | 2.94 ± 0.11 kl |
| - | 5.0 | - | - | 2.0 | 68.21 ± 1.10 j | 27.85 ± 1.16 lm | 2.74 ± 0.14 l |
Values are means ± SEM, n = 20 per treatment group. Means in a column without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the Duncan test.