Table 8.
Inter simple sequence repeat (ISSR) primers used to evaluate the genetic fidelity of ex-vitro-established micropropagated R. chalepensis plants.
| Names of Primers | Sequence 5′–3′ | Ta (°C) * | No. of Bands |
|---|---|---|---|
| UBC-825 | ACA CAC ACA CAC ACA CT | 46 | 14 |
| UBC-827 | ACA CAC ACA CAC ACA CG | 50 | 16 |
| UBC-834 | AGA GAG AGA GAG AGA GYT | 50 | 09 |
| UBC-841 | GAG AGA GAG AGA GAG AYC | 50 | 14 |
| UBC-855 | ACA CAC ACA CAC ACA CYT | 50 | 15 |
| UBC-866 | CTC CTC CTC CTC CTC CTC | 55 | 13 |
| UBC-868 | GAA GAA GAA GAA GAA GAA | 46 | 12 |
| UBC-880 | GGG TGG GGT GGG GTG | 50 | 13 |
| UBC-889 | DBD ACA CAC ACA CAC ACA C | 46 | 15 |
| UBC-891 | HVH TGT GTG TGT GTG TG | 46 | 10 |
| Total no. of bands | 131 | ||
| Average no. of bands /primers | 13.1 |
* Ta: annealing temperature.