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. 2022 May 27;20(6):356. doi: 10.3390/md20060356

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The safety evaluation of versiase. (A) The hemolysis of versiase; (B) the acute toxicity test. b1, abdominal cavity; b2, organs, three times intraperitoneal injection of versiase (400 μg) at the interval of 24 h; (C) the cytotoxicity of versiase on HUVECs; (D) live/dead fluorescent images (green: live cells, red: dead cells) of HUVECs after culturing with versiase for 12 and 24 h, and the scale bar is 100 μm. The uPA was 10,000 U. Results are presented as mean ± SD ((A–C) n = 3, D n = 6), * p < 0.05.