TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| S17-1 | thi pro hsdR hsdM+ recA RP4 2−Tc::Mu-Km::Tn7 | 51 |
| S17-1 λ-pir | S17-1 λ pir; host for pir-dependent plasmids | 51 |
| B. japonicum | ||
| UPM744 | CB1802, Hup− on soybean | 44 |
| UPM744-H series | UPM744::TnHB100 | This work |
| UPM752 | 3.15 B3, Hup− on soybean | 44 |
| UPM752-H series | UPM752::TnHB100 | This work |
| M. ciceri | ||
| UPMCa7 | Wild type; Hup− on chickpea | 33 |
| UPMCa7-H series | UPMCa7::TnHB100 | This work |
| M. loti | ||
| U226 | Wild type; Hup− on birdsfoot trefoil | 34 |
| U226-H series | U226::TnHB100 | This work |
| R. etli | ||
| CE3 | Wild type; Hup− on bean | 35 |
| CE3-H series | CE3::TnHB100 | This work |
| R. leguminosarum bv. viciae | ||
| UPM791 | 128C53 Strr | 27 |
| UML2 | Wild type; Hup− on pea | 45 |
| UML2-H series | UML2::TnHB100 | This work |
| PRE | Wild type; Hup− on pea | 29 |
| PRE-H series | PRE::TnHB100 | This work |
| S. meliloti | ||
| 102F34 | Wild type; Hup− on alfalfa | 10 |
| 102F34-H series | 102F34::TnHB100 | This work |
| Plasmids | ||
| pAL618 | pLAFR1 cosmid, hup hyp region from UPM791 | 27 |
| pCAM 140 | mTn5SSgusA40 in pUT/mini-Tn5 Spcr | 57 |
| pCE7 | pUC18 carrying DNA flanking Spcr in CE3-H7 | This work |
| pK18mobsacB | pK18 derivative, sacB Kmr | 49 |
| pKmsCE7 | pK18mobsac containing DNA flanking TnHB100 insertion in CE3-H7 and hupSL′ region | This work |
| pRL161 | pUC18 derivative containing CK1 cassette, Apr Kmr | 14 |
| pRL487 | pUC18 derivative containing CC1 cassette, Cmr Kmr | 14 |
| pRLH43 | pBS derivative carrying a 6.4-kb EcoRI DNA fragment from pAL618 | 21 |
| pUC18 | Apr, α-lac/MCS | 58 |
| pUC18Not | Apr, pUC18 with NotI sites flanking the polylinker | 20 |
| pTnB1 | pCAM140 derivative, Spcr/Apr | This work |
| pTnHB100 | pTnB1 with R. leguminosarum hup cluster, Spcr Apr | This work |