TABLE 2.
Hydrogen metabolism in bacteroids and nodules induced by TnHB100-containing rhizobial strains
| Strain | Bacteroid hydrogenase activitya | Nodule H2 evolutionb |
|---|---|---|
| B. japonicum | ||
| 752 | <10 | 4.97 ± 3.19 |
| 752-H1 | 4,400 ± 1,250 | <0.1 |
| 752-H2 | 3,200 ± 500 | <0.1 |
| 744 | <10 | 13.09 ± 1.42 |
| 744-H1 | 4,300 ± 450 | <0.1 |
| 744-H11 | 2,850 ± 250 | <0.1 |
| M. ciceri | ||
| UPM Ca7 | <10 | 4.43 ± 0.79 |
| UPM Ca7-H1 | 20 ± 10 | 3.24 ± 0.43 |
| UPM Ca7-H2 | 40 ± 10 | 4.19 ± 0.23 |
| M. loti | ||
| U226 | <10 | 5.88 ± 0.53 |
| U226-H3 | 21,300 ± 2,450 | <0.1 |
| U226-H7 | 18,075 ± 1,450 | <0.1 |
| R. etli | ||
| CE3 | <10 | 4.48 ± 2.07 |
| CE3-H2 | 10,400 ± 3,850 | <0.1 |
| CE3-H7 | 10,350 ± 3,000 | <0.1 |
| CE3-H71 | 11,075 ± 850 | <0.1 |
| R. leguminosarum bv. viciae | ||
| UPM791 | 3,000 ± 200 | <0.1 |
| PRE | <10 | 10.50 ± 3.3 |
| PRE-H4′ | 2,350 ± 500 | <0.1 |
| PRE-H2 | 2,550 ± 1,220 | <0.1 |
| UML2 | <10 | 5.49 ± 0.19 |
| UML2-H3 | 400 ± 100 | 1.85 ± 0.17 |
| UML2-H8 | 350 ± 100 | 2.31 ± 0.16 |
| S. meliloti | ||
| 102F34 | <10 | 13.24 ± 1.64 |
| 102F34-H1 | <10 | 14.24 ± 4.17 |
| 102F34-H3 | 50 ± 20 | 13.65 ± 1.74 |
a
Expressed as nanomoles of H2 consumed per hour per milligram of protein. Values are averages ± standard deviations of four determinations.
b
Expressed as micromoles of H2 evolved per hour per gram (fresh weight) of nodule.