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. 2022 May 24;14(6):1118. doi: 10.3390/pharmaceutics14061118

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Radiolabeling GMP-grade exosomes with ⁸⁹Zr. (a) Schematic of the method for labeling the surface of exosomes with ⁸⁹Zr. (b) ⁸⁹Zr-Exo size was measured using NTA shown as mean ± standard deviation (SD). (c) Zeta potential of ⁸⁹Zr-Exo was measured using Zetasizer Nano ZS shown as mean ± SD. (d) Radiochemical purity of ⁸⁹Zr-Exo was analyzed using radio-TLC. (e) In vitro cellular uptake of ⁸⁹Zr-Exo was evaluated in the THP-1 and HEK293 cell lines at the indicated time points shown as %AD ± SD.