Skip to main content
. 2022 May 24;11(6):616. doi: 10.3390/pathogens11060616

Figure 3.

Figure 3

Determination of apoptosis-like markers in T. cruzi epimastigotes subjected to nutritional stress and inhibition of AKT-like protein. Frequency histograms for Hoechst (cell cycle), DiOC6 (ΔΨm), and PI (cell membrane alteration) parameters. Flow cytometry analysis of epimastigotes grown for 24 h under standard (A) or nutritional stress (B) conditions inhibition of AKT-like protein under standard (C) or nutritional stress (D) conditions. Determination of intracellular ROS concentration in epimastigotes under standard and nutritional stress conditions with and without inhibition of the AKT-like protein for 24 h (E). Plots represent tendencies for three biological replicates per condition, and percentages are equivalent to the average obtained from three replicates. Asterisks indicate statistically significant differences with respect to the values for control treatments (*** p < 0.0004).