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. 2022 May 24;11(6):616. doi: 10.3390/pathogens11060616

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Determination of apoptosis-like in T. cruzi trypomastigotes undergoing nutritional stress and inhibition of AKT-like. The effects of AKT-like inhibition and autophagy were determined in T. cruzi trypomastigotes by flow cytometry. (A) Percentage of hypodiploidy in T. cruzi trypomastigotes under standard nutritional conditions and nutritional stress for 2 and 5 h. (B) Percentage of cell membrane damage in T. cruzi trypomastigotes under standard nutritional conditions and nutritional stress for 2 h and 5 h. (C) Frequency histogram of Ψm in T. cruzi trypomastigotes under standard nutritional conditions and nutritional stress for 2 h and 5 h. S.N.C, standard nutritional conditions; CHQ, chloroquine; S.C, conditions of nutritional stress. Asterisks indicate statistically significant differences regarding the values for control treatments (**** p < 0.0001).