TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| Mycobacterium sp. strain PYR-1 | Mineralizes high-molecular-weight PAHs such as fluoranthene, pyrene, and phenanthrene | 8 |
| Escherichia coli XL1-Blue MRF′ | lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| Escherichia coli XLOLR | lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] Su− (nonsuppressing) λr | Stratagene |
| Escherichia coli TOP10 | One Shot competent cells, F′ mcrA Δ(mmr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ(ara-leu)7697 | Invitrogen |
| Plasmid or vector | ||
| ExAssist helper phage | For in vivo excision of the pBK-CMV phagemid from the ZAP Express vector with E. coli XLOLR | Stratagene |
| Zap Express vector | Lambda vector, prokaryotic and eukaryotic expression, in vivo excision of the pBK-CMV vector | Stratagene |
| pBK-CMV vector | Neor Kanr, ColE1 origin, lacZ, cytomegalovirus promoter | Stratagene |
| Myco-pBK-CMV phagemid | pBK-CMV phagemid with 1.8-kb insert from Mycobacterium sp. strain PYR-1 | This study |
| pBAD/Thio-TOPO vector | Ampr pMB1 origin, six-His-Thioredoxin open reading frame, arabinose induced | Invitrogen |
| katG-pBAD/Thio plasmid | pBAD/ThioFusion with the katG gene of Mycobacterium strain PYR-1 | This study |