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Reversed-phase HPLC-PDA chromatograms of soaked scaffolds. PDA parameters: 200–350 nm. (A) Elution profile of the chemical standards (Sigma): PA, phthalic acid; 4ABP, 4-aminobiphenyl; KGN, kartogenin. (B) PBS blank extract (negative control). (C) The 12% scaffold, day 2 extract. For the day 2 extracts, KGN accounted for >90% of the peaks detected by the PDA detector. Details of the extraction procedure are described in the Materials and Methods.
