Table 1.
Fatty acid composition determined by GC–MS of the lipid extracts obtained from Nannochloropsis gaditana comparing different extraction techniques.
| Fatty Acid | RT (min) | % Fatty Acids | |||||
|---|---|---|---|---|---|---|---|
| Folch | Control | Viscozyme | Celluclast | Saczyme | Mix | ||
| 12:0 | 6.2 | 0.4 ± 0.0 | 0.6 ± 0.1 | 0.5 ± 0.0 | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.6 ± 0.0 |
| 14:0 | 10.0 | 4.3 ± 0.1 | 4.5 ± 0.1 | 4.3 ± 0.1 | 4.4 ± 0.1 | 4.3 ± 0.2 | 4.4 ± 0.1 |
| 16:1 n-7 | 14.6 | 26.1 ± 0.6 a | 22.9 ± 0.8 b | 24.6 ± 0.7 a,b | 24.0 ± 0.8 a,b | 24.9 ± 1.1 a,b | 23.2 ± 0.6 b |
| 16:0 | 15.1 | 23.0 ± 0.8 a | 18.3 ± 1.3 b | 21.7 ± 1.3 a,b | 19.7 ± 1.6 a,b | 21.5 ± 1.7 a,b | 19.1 ± 0.7 a,b |
| 17:1 | 16.3 | 0.7 ± 0.0 | 0.7 ± 0.2 | 0.5 ± 0.2 | 0.7 ± 0.1 | 0.5 ± 0.3 | 0.3 ± 0.1 |
| 18:2 isomer n.i. | 17.1 | 0.7 ± 0.1 | 0.5 ± 0.2 | 0.7 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0.0 | 0.7 ± 0.0 |
| 18:2 n-6 | 19.7 | 3.2 ± 0.1 b | 3.5 ± 0.0 a | 3.5 ± 0.0 a | 3.5 ± 0.0 a | 3.5 ± 0.0 a | 3.5 ± 0.0 a |
| 18:1 n-9 | 19.8 | 4.8 ± 0.1 | 4.7 ± 0.0 | 4.7 ± 0.0 | 4.8 ± 0.0 | 4.8 ± 0.0 | 4.7 ± 0.1 |
| 18:1 n-7 | 20.0 | 0.7 ± 0.1 | 0.6 ± 0.0 | 0.5 ± 0.1 | 0.6 ± 0.0 | 0.5 ± 0.1 | 0.5 ± 0.0 |
| 18:0 | 20.6 | 0.2 ± 0.0 a | 0.2 ± 0.0 b | 0.2 ± 0.0 b | 0.2 ± 0.0 b | 0.2 ± 0.0 b | 0.2 ± 0.0 b |
| 20:4 n-6 | 24.2 | 5.9 ± 0.7 b | 7.1 ± 0.0 a | 6.8 ± 0.1 a,b | 7.0 ± 0.2 a | 6.8 ± 0.2 a,b | 7.3 ± 0.1 a |
| 20:5 n-3 | 24.5 | 29.3 ± 0.8 | 34.5 ± 1.9 | 30.6 ± 1.9 | 32.7 ± 2.1 | 30.2 ± 2.4 | 33.8 ± 1.1 |
| 20:5 isomer n.i. | 24.6 | 0.6 ± 0.2 | 0.9 ± 0.1 | 1.1 ± 0.1 | 1.1 ± 0.2 | 1.1 ± 0.5 | 1.2 ± 0.4 |
| 20:5 isomer n.i. | 25.8 | n.d. | 1.1 ± 0.1 | 0.6 ± 0.2 | 0.7 ± 0.4 | 0.6 ± 0.2 | 0.4 ± 0.2 |
| SFA | 27.9 ± 1.0 a | 23.5 ± 1.3 b | 26.5 ± 1.4 a,b | 24.7 ± 1.7 a,b | 26.4 ± 1.9 a,b | 24.3 ± 0.8 a,b | |
| MUFA | 32.4 ± 0.9 a | 28.9 ± 1.2 b | 30.4 ± 1.1 a,b | 30.0 ± 1.1 a,b | 30.8 ± 1.7 a,b | 28.8 ± 1.2 b | |
| PUFA | 39.7 ± 2.0 b | 47.6 ± 2.5 a | 43.1 ± 2.5 a,b | 45.6 ± 2.8 a,b | 42.8 ± 3.6 a,b | 47.0 ± 1.7 a | |
| n-3 | 29.3 | 34.5 | 30.6 | 32.7 | 30.2 | 33.8 | |
| n-6 | 9.1 | 10.6 | 10.3 | 10.5 | 10.2 | 10.8 | |
| n-6/n-3 ratio | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | |
RT, retention time; SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; n.i., not identified; n.d., not detected. Results expressed as percentage over the total content (relative content). Values are the mean ± SD of three determinations. Different letters indicate statistically significant differences between extraction methods at p < 0.05 (one-way ANOVA with post-hoc Tukey, a–b).