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Recognition of PspA4Pro released from NPs by anti-PspA antibodies. (a) PGA-co-PDL and (c) PLGA NPs with or without chitosan prepared at pH 4.0 or 7.0 by o/w single emulsion with surface-adsorbed PspA4Pro. (b) PGA-co-PDL and (d) PLGA NPs prepared at pH 7.0 by w/o/w double emulsion with encapsulated PspA4Pro. Chitosan hydrochloride (HCl-CS) or carboxymethyl chitosan (CM-CS) were employed. PspA4Pro was used as a positive control. Data represent mean ± SD, n = 3.
