Table 4.
Effect of pH and chitosan type on PspA4Pro adsorption and encapsulation into PGA-co-PDL and PLGA hybrid NPs prepared by single (o/w) or double (w/o/w) emulsion solvent evaporation method (mean ± SD, n = 3).
| PGA-co-PDL | PLGA | ||||
|---|---|---|---|---|---|
| Formulation | PspA (µg)/NP (mg) | %LE * | PspA (µg)/NP (mg) | %LE * | |
| Adsorption | without chitosan pH 7.0 | 25.57 ± 8.13 | 63.94 ± 20.33 | 8.24 ± 6.28 | 20.60 ± 15.7 |
| without chitosan pH 4.0 | 9.31 ± 4.34 | 23.26 ± 10.85 | 36.28 ± 0.23 | 90.69 ± 0.57 | |
| HCl-CS pH 7.0 | 36.49 ± 1.15 | 91.23 ± 2.87 | 37.59 ± 1.94 | 93.97 ± 4.84 | |
| CM-CS pH 7.0 | 15.41 ± 2.48 | 38.52 ± 6.21 | 10.55 ± 5.10 | 26.40 ± 12.62 | |
| CM-CS pH 4.0 | 11.39 ± 4.13 | 28.49 ± 10.33 | 29.62 ± 7.80 | 74.04 ± 19.48 | |
| Encapsulation | without chitosan pH 7.0 | 4.14 ± 0.22 | 2.07 ± 0.80 | 29.57 ± 4.9 | 14.78 ± 7.36 |
| HCl-CS pH 7.0 | 22.88 ± 2.65 | 11.44 ± 6.62 | 59.33 ± 1.32 | 29.66 ± 4.65 | |
| CM-CS pH 7.0 | 7.86 ± 1.83 | 3.93 ± 6.71 | 11.88 ± 4.15 | 5.94 ± 1.7 | |
* %LE = load efficiency.