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. 2022 Jun 10;12:865528. doi: 10.3389/fcimb.2022.865528

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Copyright © 2022 Mo, Xu, Rosa, Hasan and Adams

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantifying AJ health during S. pneumoniae infection. Representative images of (A) untreated, (B) low-dose infection, and (C) high-dose infection monolayers, respectively. (D) IJOQ analysis or (E) TiJOR analysis of untreated or infected monolayers (n = 3). Asterisk(s) indicate intercellular junction organization is significantly greater with mock infection or 1 x 10⁶ as determined by one-way ANOVA with a post hoc Tukey test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, not significant.