TABLE 2.

Effect of emulsifiers on reutericyclin production

Source of fatty acids added before or after growth	Inhibitory activity (AU ml−1) of NCS prepared froma:
	Culture in mMRS4 with Tween 80 replaced by emulsifiers	Culture in mMRS4 (oleate), emulsifiers added after growth
Oleate (control)	4.5 ± 0.9	6.3 ± 2.3
Lamesorb SMO	10 ± 7.3	5.2 ± 0.7
Lamegin GLO30	85 ± 38	16 ± 5.7
Lamegin ZE 609 O18	7.8 ± 1.1	6.2 ± 2.6
Tween 80	86 ± 21	14 ± 10
Wheat germ oil	7.8 ± 2.8	NDb

^aShown is the mean ± standard deviation of two independent experiments. 

^bND, not determined.