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Purification protocol of reutericyclin
| Purification step | mMRS4 (oleic acid)
|
mMRS4
|
||||
|---|---|---|---|---|---|---|
| Vol (ml) | Total activity (103 AU) | Yield (%) | Vol (ml) | Total activity (103 AU) | Yield (%) | |
| Cell extract | 500 | 150 | 100 | 500 | 100 | 100 |
| Gel filtration | 1 | 120 | 80 | 1 | 70 | 70 |
| RP-FPLC | 1 | 90 | 60 | 1 | 72 | 72 |
| RP-HPLC | 0.5 | 70 | 50 | 0.5 | 23 | 23 |
