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Testing of German negative horse populations and provisional cut-off determination. A total 288 HeV-negative German horse sera were tested in HeV-G and HeV-N ELISA, and the mean PP values plus 3 SD was set as a provisional cut-off. For HeV-G the initial cut-off PP value was determined as 26 (A) and for HeV-N as 39 (B), as shown by the red dotted lines.
