Table 3.
Test results of ring trial samples in HeV-G ELISA. When applying the cut-off PP values based on ROC analysis with the highest combined DSe and DSp of 40, two dilutions of positive serum samples were identified as questionable and would be repeated by Western Blot or IF analysis.
| Sample ID | Sample Information | PP | Result | Interpretation |
|---|---|---|---|---|
| neg control | 8.55 | neg | ||
| pos control | 100.00 | pos | ||
| RTS 1 | infected horse number 1; 1/100 | 47.72 | pos | correct |
| RTS 2 | vaccinated horse | 76.57 | pos | correct |
| RTS 3 | infected horse number 1; 1/150 | 35.28 | neg | questionable |
| RTS 4 | infected horse number 2; 1/60 | 43.59 | pos | correct |
| RTS 5 | negative horse | 8.21 | neg | correct |
| RTS 6 | infected horse number 2; 1/40 | 42.36 | pos | correct |
| RTS 7 | infected horse number 2; 1/60 | 31.43 | neg | questionable |
| RTS 8 | infected horse number 2; 1/40 | 59.40 | pos | correct |
| RTS 9 | negative horse | 10.25 | pos | correct |