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. 2000 Oct;66(10):4334–4339. doi: 10.1128/aem.66.10.4334-4339.2000

FIG. 1.

FIG. 1

Detection of algicidal activity. Algicidal activity was detected as described in Materials and Methods. Zones of clearing around paper disks indicate the lysis of the diatom S. costatum strain NIES-324. (A) Algicidal activity in A28 culture supernatants and samples from each of the purification steps. a, A28 culture supernatant (20 μl [0.16 U of protease activity]); b, fresh ASWM medium (20 μl); c, heated culture supernatant (20 μl); d, culture supernatant concentrated by ultrafiltration and reconstituted with fresh ASWM medium (0.16 U of protease activity); e, ultrafiltrate of culture supernatant (20 μl); f, protease I-rich fraction from a Poros HQ/M anion-exchange chromatography (0.16 U of protease activity); g, protease II-rich fraction from a Poros HQ/M anion-exchange chromatography (0.16 U of protease activity); h, protease I-rich fraction from preparative native-protein gel electrophoresis (0.16 U of protease activity). (B) Algicidal activity with Pseudoalteromonas strains A28, NH1, and NH2.