FIG. 3.

Elution profiles of the protease activities of concentrated culture supernatants of Pseudoalteromonas sp. strain A28 from a Poros HQ/M anion-exchange column. The column was developed with a linear NaCl gradient of 0 to 1 M in Tris buffer (10 mM, pH 8.0). Protease activity was detected using the azocasein assay as described in Materials and Methods.