TABLE 2.
Purification of protease I from the culture supernatant of Pseudoalteromonas sp. strain A28
| Sample analyzed | Total protein (mg) | Total activity (U) | Sp act (U mg of protein−1) | Purificationa (fold) | Yield (%) |
|---|---|---|---|---|---|
| Culture supernatant | 613 | 1,701 | 3 | 1 | 100 |
| Concentrated supernatantb | 41.6 | 1,586 | 38 | 13 | 93 |
| Poros HQ/M column peak I | 3.0 | 496 | 165 | 55 | 29 |
| Preparative electrophoresis peak | 0.9 | 184 | 204 | 68 | 11 |
a
Calculated from the specific activities.
b
Concentrated by ultrafiltration with a 10,000-Mw-cutoff membrane.