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. 2000 Oct;66(10):4340–4344. doi: 10.1128/aem.66.10.4340-4344.2000

FIG. 1.

FIG. 1

(A) Direct ITS-PCR amplification from broth cultures and colonies. Lanes: 1, 3, and 5, Y. lipolytica strain 160; 2, 4, and 6, C. zeylanoides strain 155; 1 and 2: 36-h TGY broth culture; 3 and 4, 12-h TGY broth culture; 5 and 6, 36-h colony on TGY agar plate; M, DNA size marker. (B) Effect of cell concentration on amplification by direct ITS-PCR from 48-h TGY broth culture of Y. lipolytica strain 160. Cell populations (CFU per milliliter): lane 1, 3 × 108; lane 2, 3 × 106; lane 3, 3 × 104; lane 4, 3 × 103.