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. 2022 May 27;10(6):288. doi: 10.3390/toxics10060288

Table 1.

Selected sequential extraction procedure for As fractioning.

Step Extractant Time/Temperature As Fraction Reference
1 (NH4)2SO4 0.05M 4 h/25 °C Easily exchangeable As [57]
2 (NH4)H2PO4 0.05M 16 h/20 °C Specifically adsorbed As [57]
3 Acetic buffer 1M (pH 5) 12 h/25 °C As bound with calcite [61]
4 NH4-oxalate buffer 0.2M (pH 3.2) 4 h/20 °C + 10 min (wash) Low crystalline Fe(III)-oxyhydroxides [57]
5 NH4-oxalate buffer 0.2M + ascorbic acid 0.1M (pH 3.2) 30 min/96 °C + 10 min (wash) Crystalline Fe(III)-oxides [57]
6 HNO3 8M 3 h/80 °C Sulfides [84]
7 Microwave digestion (HNO3 + H2O2) 30 min/180 °C Residual fraction [57]