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. 2022 May 30;14(6):1172. doi: 10.3390/pharmaceutics14061172

Figure 2.

Figure 2

The microarray analysis of miRNAs for endometrial tissue. Total RNA of endometrial stromal cells was purified. The miRNA expression profiles were analyzed using the Affymetrix Gene-Chip miRNA 4.0 array. (A) Differential expression of miRNAs between normal pregnancy and spontaneous miscarriage in a hierarchical clustering analysis. The red color indicates up-regulation, and the green color indicates down-regulation. To identify miRNA expression signatures, we performed miRNA microarray analysis in endometrial tissue. Hierarchical clustering analysis revealed a significant down-regulation of 8 miRNAs and up-regulation of 7 miRNAs in spontaneous miscarriage ESC compared to normal pregnancy ESC. (B) Volcano plot of microarray data. Plot shows differences between normal pregnancy and spontaneous miscarriage. Differentially expressed miRNAs with a fold change ≥1.5 and p < 0.05 between the two groups are shown in red and green. (C) RT-qPCR analysis of the expressed miRNA. Total extracellular vesicle RNAs were reverse transcribed to cDNA with primers for miR-138-5p and U6 small nuclear RNA. miR-138-5p is down-regulated in spontaneous miscarriage compared to normal pregnancy.