Table 2.
Characterization of canine mesenchymal stromal cells cultured in αMEM.
| Culture media | Tissue source | Seeding density | Surface markers | Tri-lineage differentiation potential | References |
|---|---|---|---|---|---|
| α-MEM+ 10% FBS + 1%pen/strep + 0.05% Fungizone + 0.1 mM ascorbic acid-2-phosphate + 1.00E-0.9M dexamethasone + 1 ng/mL bFGF | BM LV |
6x103cells/cm2 | CD105(+) CD90(+) CD29(+) CD166(+) CD45(−) | AD-CH-OST | (166) |
| αMEM + 10%FBS + 80 μg/mL gentamicin | AT-OM AT-SUBC |
5x103cells/cm2 | CD90(+) CD44(+) CD45(−) CD11b(−) | AD-CH-OST | (101) |
| αMEM + 10%FBS + 100 U/ml pen + 100 μg/ml strep | SY BM AT |
1x102cells/cm2 | CD90(+) CD44(+) CD9(+) CD105(+;−) CD45(−) CD34(−) STRO-1(−) | AD-CH-OST | (167) |
| αMEM + 20%FBS + 100 U/ml pen + 100 mg/ml strep sulfate + 2 mM glutamine | B-MAN B-FEM |
NK | NK | AD-CH-OST | (168) |
| αMEM + 15%FBS+1% pen/strep | AM | NK | CD90(+) CD105(+) CD45(−) CD34(−) | AD-CH-OST | (169) |
αMEM, Alpha Modified Eagle's Medium; FBS, fetal bovine serum; bFGF, basic fibroblast growth factor; Pen, penicillin; Strep, streptomycin; CD, cluster of differentiation; (+), positive expression; (−), negative expression; (+;-), variable expression due to tissue origin; AT, adipose tissue; LV, liver; AT-OM, adipose tissue omentum; AT-SUBC, subcutaneous adipose tissue; SY, synovium; B-MAN, mandible; B-FEM, femur; AM, amnion; AD, adipogenic differentiation; CH, chondrogenic differentiation; OST, osteogenic differentiation; NK, not known.