Table 4.
Characterization of canine mesenchymal stromal cells cultured in serum-free media.
| Culture media | Tissue source | Seeding density | Surface markers | Tri-lineage differentiation potential | References |
|---|---|---|---|---|---|
| Commercial SFM-StemPro® | AT | 5 × 103 cells /cm2 | CD90(+) CD44(+) CD54(+) CD45(–) CD34(–) MHC–II(–) | / | (151) |
| In-house SFM | AT | 3 × 105 cells /cm2 | CD105(+) CD90(+) CD14(–) CD34(–) | AD-CH-OST | (176) |
| In-house SFM and Commercial SFM—RoosterBio | AT | 3 × 105 cells /cm2 | CD44(+) CD90(+) | AD-CH-OST | (150) |
SFM, serum-free media; AT, adipose tissue; CD, cluster of differentiation; AD, adipogenic differentiation; CH, chondrogenic differentiation; OST, osteogenic differentiation; (+), positive expression; (–), negative expression; /, not assessed; NK, not known.