FIG. 3.
Survivor curves for thermal inactivation of L. monocytogenes cells at 60°C. All cells were grown and inactivated as described in Materials and Methods. (a) L. monocytogenes cells grown in brain heart infusion at 37°C (□), L. monocytogenes cells grown in brain heart infusion at 37°C and cold shocked to 0°C for 3 h (■), L. monocytogenes cells grown in Pine's medium at 37°C (○), L. monocytogenes grown in Pine's medium at 37°C and cold shocked to 0°C for 3 h (●). (b) L. monocytogenes cells grown in brain heart infusion at 37°C (□), L. monocytogenes cells grown in brain heart infusion at 37°C and cold shocked to 0°C for 3 h (■), L. monocytogenes cells grown in brain heart infusion at 37°C and treated with kanamycin (310 μM) (▵), L. monocytogenes cells grown in brain heart infusion at 37°C and treated with tetracycline (310 μM) (◊).