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. 2022 Jun 14;14(6):1304. doi: 10.3390/v14061304

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Viral NA enzyme kinetics. To determine the viral NA enzyme activities in real-time, the emission signals from different concentrations of MUNANA cleaved by NA of (A) RG pH1N1, (B) RG pH1N1(I365T/S366N), (C) RG pH1N1(I365E/S366D), and (D) RG pH1N1(I365T/S366A) were measured every 60 s for 1 h. (E) Michaelis–Menten plots of viral NA enzyme activities. (F) Lineweaver–Burk plots of viral NA enzyme activity. (G) Vmax, (H) Km, and (I) Kcat/Km of each viral NA calculated using Lineweaver–Burk plots. Statistical tests for multiple comparisons in (G–I) were analyzed using one-way ANOVA with Tukey’s or Holm–Sidak’s multiple comparison tests. (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001). Error bars are plotted as standard deviation from the mean value.