TABLE 3.
Fungal community analysis for two avocado grove soils
| Identity of OTU (GenBank accession no.)a | Cloneb | No. of clones identified in:
|
|
|---|---|---|---|
| Powell soil | Vanoni soil | ||
| Tritirachium sp. (AB003951) | JK2-2 (99) | 11 | 13 |
| Pleospora rudis (U00975) | JK2-4 (97) | 9 | 2 |
| Petriella setifera (U43908) | JK3-18 (98) | 6 | 2 |
| Aspergillus versicolor (AB008411) | JK2-9 (99) | 2 | 5 |
| Chromocleista cinnabarina (AB006747) | JK2-22 (99) | 3 | 1 |
| Monilinia laxa (Y14210) | JK2-17 (96) | 1 | 2 |
| Exophiala jeanselmei (X80705) | JK3-21 (96) | 0 | 2 |
| Nectria cinnabarina (AB003949) | JK3-36 (98) | 0 | 1 |
| Verticillium dahliae (U33637) | JK3-30 (99) | 0 | 1 |
| Coccodinium bartschii (U77668) | JK3-25 (97) | 0 | 1 |
a
Fungal rDNA clone libraries from each soil were constructed by performing PCRs with the nu-SSU-0817-5′ and nu-SSU-1536-3′ primers. rDNA clones from each soil were randomly selected and sorted into OTUs by restriction enzyme analysis. The nucleotide sequence of one representative clone from each OTU was determined and analyzed by using BLAST (NCBI) and Gap (Genetics Computer Group) to identify the database sequence most similar to the clone and to obtain a phylogenetic identity for the OTU.
b
The numbers in parentheses are the percentages of similarity of the rDNA clones to the OTU sequences.