Figure 2.

Syrosingopine reduces the extracellular acidification rate (ECAR) and decreases intracellular pH in cancer cells. Glucose-dependent ECAR was determined using the Seahorse XF96 bioenergetic analyzer in MDA-MB-231 (A) and FaDu cells (C) after 24 h of treatment with increasing concentrations of syrosingopine. Intracellular pH values were measured in MDA-MB-231 (B) and FaDu cells (D) after 72 h of treatment with increasing concentrations of syrosingopine. Data are represented as mean ± SEM of at least three independent experiments. Significance was determined using one-way or two-way ANOVA: * p < 0.05; ** p < 0.01; *** p < 0.001; # = **** p < 0.0001. ns = not significant.