Figure 4.

Impact of syrosingopine on tumor cell viability and proliferation. MDA-MB-231 (top, A–C) and FaDu (bottom, D–F) cells were treated with syrosingopine for 24, 48 and 72 h. Cell viability (A,D) and proliferation (B,E) are represented after 72 h of treatment. Percentages of early apoptotic/necrotic cells were calculated after treatment with 25 µM of syrosingopine for 72 h (C,F). Data are represented as mean ± SEM of at least three independent experiments. Significance was determined using one-way or two-way ANOVA: * p < 0.05; *** p < 0.001; # = **** p < 0.0001. ns = not significant.