Figure 6.

RNase activity in blank solution (300 µg/mL in 0.01 M PBS pH 7.2) and in solution containing 1 mg/mL beta-casein after ultrafiltration through a membrane filter with a pore diameter of 30 kDa (A) and time-course of binase release from alginate hydrogel and alginate hydrogel supplemented by beta-casein (B). 100% of RNase activity of the loaded protein (calculated as difference between the activity of initial solution in 0.01 M sodium phosphate buffer and resting activity after hydrogels incubation) was taken.