FIG. 1.

Maps of ς³² antisense expression plasmid pSE420αs (A) and OPH-ς³² antisense expression plasmid pTOas (B). Antisense was inserted into pSE420αs between NcoI and HindIII restriction sites under control of the trc promoter. For the pTOas plasmid, trc-ς³² antisense fragment from pSE420αs was engineered with NdeI restriction enzyme sites and inserted into a plasmid containing the OPH gene (pTO), also under trc control.