FIG. 2.

Northern blot analyses of ς³² antisense mRNA. (A) Lanes 1, 3, 5, 7, and 9 are time course samples from unstressed cultures to which no IPTG has been added (no induction of ς³² antisense mRNA). Lanes 2, 4, 6, 8, and 10 are time course samples from unstressed cultures that were induced for ς³² antisense mRNA synthesis by addition of 1 mM IPTG at 0 min. All quantified values were normalized to the value of lane 4 (10 min). (B) Lanes 1, 3, 5, 7, and 9 are time course samples from cultures that were ethanol stressed (4%, vol/vol) at 0 min but to which no IPTG has been added (Uninduced). Lanes 2, 4, 6, 8, and 10 are time course samples from cultures that were both ethanol stressed and induced for antisense synthesis (Induced). All quantified values were normalized to the value of lane 4. Error bars in both panels represent standard error from multiple blots from multiple experiments.