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. 2022 Jun 11;14(6):1277. doi: 10.3390/v14061277

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic tree of different TTV types. The tree includes (i) cloned and sequenced TTVs from the children (the number in parentheses depicts how many clones from the sample had the same sequence), (ii) all TTV-type species detected in humans, and (iii) all TTV clones/plasmids used in this study as controls for method development (marked as pXXX in the tree; see also Section 2.5.5). GenBank number of the previously published sequences is mentioned on the tree. The tree is made based on the ~500 nt region of ranging from the conserved UTR to close to the end of ORF2 (the multiplex PCR amplicon area). The phylogenetic tree was generated in MEGA X using the maximum likelihood method and general time reversible model, and bootstrap values were determined by 1000 replicates. Branch support values are given when >70.