Table 2.
Comparison of consensus sequences generated from the same sample using the amplicon-based or shotgun metagenomic approach and comparison to the reference genome with accession number NC_001672.1. (Abbreviations: aa = amino acids, bp = base pairs, UTR = untranslated region, Ampli = amplicon-based sequencing, Meta = shotgun metagenomic sequencing).
Length of Sequence | Length of Polyprotein | Length of UTR (5′ URT/3′ UTR) [bp] | GC Content | |||||
---|---|---|---|---|---|---|---|---|
Ampli | Meta | Ampli | Meta | Ampli | Meta | Ampli | Meta | |
Reference NC_001672.1 |
11,141 | 10,245 bp /3414 aa |
131/764 | 54% | ||||
2008-P2-S1/S2 | 11,142 | 11,091 | 10,245 bp /3414 aa |
10,245 bp /3414 aa |
131/766 | 131/715 | 54% | 54% |
2019-P18-S1/S2 | 11,009 | 11,005 | 10,245 bp /3414 aa |
10,245 bp /3414 aa |
87/673 | 87/670 | 53% | 53% |
2020-P19-S1/S2 | 11,008 | 11,041 | 10,245 bp /3414 aa |
10,245 bp /3414 aa |
73/730 | 106/690 | 54% | 54% |
2020-P21-S1/S2 | 11,009 | 11,057 | 10,245 bp /3414 aa |
10,245 bp /3414 aa |
87/677 | 111/701 | 54% | 54% |