Figure 3.

Bradykinin and EGF treatments show increased viral binding. L1 viral capsid protein was measured via western blot after the incubation of 200 ng/mL bradykinin and EGF. PsVs were added for 0 min or 2 h with samples incubated with or without drug on ice. HaCaT cells were washed three times with 1× PBS and then harvested with trypsin. (A, 1st lane) control sample without PsVs and drug, (A, 2nd lane) control sample with virus that were immediately washed off, (A, 3rd and 4th lane) control infection after 2 h, (A, 5th and 6th lane) cells treated with bradykinin and virus for 2 h, (A, 7th and 8th lane) cells treated with EGF and virus for 2 h. (B) Densitometry data of western blot in A showing the relative intensity of L1 protein normalized to actin for two samples from different wells for each treatment with either virus or virus and drug.