Figure 5.

ML-141 treatment reduces viral binding. L1 viral capsid protein was measured via western blot after the incubation of 10 µM ML-141. HaCaT cells were treated with ML-141 for 2 h prior to viral addition at 37 °C. PsVs were added for 0 min or 2 h with samples incubated with or without the drug on ice. HaCaT cells were washed three times with 1× PBS and then harvested with trypsin. (A, 1st lane) control sample without PsVs and drug, (A, 2nd lane) control sample with virus that was immediately washed off, (A, 3rd and 4th lane) control infection after 2 h, (A, 5th and 6th lane) cells treated with ML-141 and virus for 2 h. (B) Densitometry data of western blot in A showing the relative intensity of L1 protein normalized to actin for two samples from different wells treated with either virus or virus and 10 µM ML-141.