Table 3.
The design of primers.
| Gene | Upstream Primers (5′ to 3′) | Downstream Primers (5′ to 3′) |
|---|---|---|
| GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATCATTCTCATGG |
| Actin | CCGTCTTCCCCTCCATCGT | ATCGTCCCAGTTGGTTACAATGC |
| MT-MMP-1 | GGTGGAGGTTGTAGGTGTGA | CAACCCTCAGAGAGCAAAGC |
| CDK2 | CCAAGTGAGACTGAGGGTGT | CCAAGTGAGACTGAGGGTGT |
| CDK4 | CTGCAGGCTCATACCATCCT | ACTCTTGAGGGCCACAAAGT |
| Cyclin D1 | CTCCTTTCTCCACCCACCTC | TCCTCTGCTGGACACCCC |
| Cyclin E1 | CAGCGGTTGTAATGTGACCC | AAAGCTCTTCCCCACCCAAT |
| CXCL8 | TGTCCTATTGAGAACCACGGT | GCAAGCTAAGACTCTCCAGC |
| p21 | CCCAAGCTCTACCTTCCCAC | CTGAGAGTCTCCAGGTCCAC |
| IL6 | TCCACTGGAATTTGCTTGCC | AGTGCCTCTTTGCTGCTTTC |
| FASN | TGTGGTGTGTGGGTTGGTAT | GGACGAAATGGGGATAGCCT |
| Snail | CTACCTGTTTGCACACTCGG | ATTTGGTCTTGGCAAAAGCC |
| Slug | TTCTACGTTCTCTGGGCTGG | ACCCAGGCTCACATATTCCT |
| E-cadherin | AACGCATTGCCACATACACTC | GACCTCCATCACAGAGGTTCC |
| Vimentin | TCAATGTTAAGATGGCCCTTG | TGAGTGGGTATCAACCAGAGG |