FIG. 2.
Titration of internal control RNAs by RT-PCR in the presence of Pan-1 RNA. Serial dilutions of internal RNA controls (10−6 to 10−8) and Pan-1 RNA (10−5 to 10−7) were tested separately (lanes a1 through a3, Pan-1 RNA diluted 10−5, 10−6, and 10−7, respectively; lanes e1 through e3, control RNAs H and B diluted 10−6, 10−7, and 10−8, respectively) or with combinations of different dilutions of RNAs. Lanes b1 through b3 contained 10−6 dilutions of control RNAs H and B and 10−5, 10−6, and 10−7 dilutions of Pan-1 RNA, respectively; lanes c1 through c3 contained 10−7 dilutions of control RNAs H and B and 10−5, 10−6, and 10−7 dilutions of Pan-1 RNA, respectively; and lanes d1 through d3 contained 10−8 dilutions of control RNAs H and B and 10−5, 10−6, and 10−7 dilutions of Pan-1 RNA, respectively. Lane M contained a 1-kb DNA marker. Lanes N and P contained negative and positive controls for RT-PCR.